Stacy, Dana, and Dave recently presented at the SEED conference in Boston last week. It was a great opportunity to hear about cutting edge research in Synthetic Biology, see friends, and meet new colleagues.
And then there were three! A big congrats to Ben Oakes, who passed his PhD qualifying exam with flying colors!
Rob Nichols, a first year student from the MCB graduate program, has decided to join the lab. Rob will work on the basic science of cyanobacteria regulate and catalyze carbon assimilation.
A note from the instructors of this year's CSHL Synthetic Biology course:
We the instructors for the third annual Cold Spring Harbor Summer Course on Synthetic Biology are excited to announce that we are accepting applications for 2015! This two-week immersive course in synthetic biology has received enthusiastic reviews from past students. We encourage you to apply if…
- You are a scientist whose training is well underway (senior graduate student to junior faculty and beyond).
- You are interested in steering your research in a new direction, towards synthetic biology.
- You are interested in a multi-disciplinary approach to biology and bioengineering. We encourage students of all backgrounds, whether the very biological or very theoretical, to apply.
Applications are due April 15, 2015
For details on our course and and how to submit your application, visit: http://meetings.cshl.edu/courses/2015/c-synbio15.shtml
Learn Techniques and Perform Research at the Forefront of Synthetic Biology: The course will focus on how the complexity of biological systems, combined with traditional engineering approaches, results in the emergence of new design principles for synthetic biology. The Course centers around an immersive laboratory experience. Here, students will work side-by-side with instructors in teams to learn the practical and theoretical underpinnings of cutting edge research in the area of Synthetic Biology. Broadly, we will explore how cellular regulation- transcriptional, translational, post-translational and epigenetic- can be used to engineer cells to accomplish well-defined goals. Specific laboratory modules will cover the following areas: cell-free transcription and translation systems, high-throughput cloning techniques, computational biology using ordinary differential equations models, biosensor development for metabolic engineering, and CRISPR for genome editing in mammalian cells to regulate synthetic genes and physical cell properties. Students will first learn essential synthetic biology techniques in a four-day ‘boot-camp’, and then rotate through research projects in select areas.
In addition, students will interact closely with a panel of internationally-recognized speakers who will give students a broad overview of applications for synthetic biology, including renewable chemical production and therapeutics, the current state-of-the-art techniques, and case studies in human practices and socially responsible innovation. For 2015 these speakers include: Pam Silver, Tom Knight, Elisa Franco, Danielle Tullman-Erceck, Jeff Tabor, Dave Savage, Vincent Noireaux, and several others.
Congratulations to Rayka on her new paper in Photosynthesis Research describing our methodologies for imaging cyanobacteria. This paper gives context to the potential for live-cell imaging of cyanobacteria and also goes into great detail on all of our 'trade secrets' for how we carry out our experiments. We hope the field finds it useful!
Yokoo, R., Hood, R. D., & Savage, D. F. (2014). Live-cell imaging of cyanobacteria. Photosynthesis Research. doi:10.1007/s11120-014-0049-x
A big congratulations to Ben for his recent paper describing our initial efforts at engineering the RNA-directed DNA nuclease Cas9. This paper describes some of Ben's initial work at constructing genetic screens to interrogate and engineer Cas9 function. Hopefully this will be the start of many interesting things to come!
Oakes, B. L., Nadler, D. C., & Savage, D. F. (2014). Protein engineering of Cas9 for enhanced function. Methods in Enzymology, 546, 491–511. doi:10.1016/B978-0-12-801185-0.00024-6
Dave and Rayka just finished up another successful summer of instructing at the Cold Spring Harbor Laboratory course on Synthetic Biology! The course is an immersive, laboratory based approach for exposing students to the practical and theoretical underpinnings of Synthetic Biology. It was developed by Dave and three faculty colleagues, Karmella Haynes (ASU), Julius Lucks (Cornell), and Jeff Tabor (Rice) and had it's inaugural debut in 2013. More details can be found at:
A big (belated) welcome to new graduate students Sean, Ben, and Avi, who are joining the lab from the MCB PhD graduate program!
This summer, Dave will be teaching a course on Synthetic Biology at the Cold Spring Harbor Laboratory. This course is aimed at developing a core curricula in the area and for bringing new graduate students and postdoctoral fellows into the field. You can read more about it at CSHL and the 2014 Course website:
If you are interested in applying, please contact Dave!
Dave was recently selected as a 2013 New Innovator by the National Institutes of Health! This 2.5 million dollar award will enable our work in constructing novel fluorescent biosensors to measure the metabolism of cellular disease states. More information can be found at:
A huge congratulations to postdoctoral fellow Rayka Yokoo, who was recently selected as a 2013 Life Sciences Research Foundation Fellow of the Simons Foundation. Nice work! More details can be found at their respective websites:
A big congratulations to Benz, who recently passed his PhD qualifying exam in the Chemistry graduate program. Onwards and upwards!
A huge kudos to Caleb and Rachel for passing their MCB Qualifying Exams with flying colors! Next stop,[gallery link="file" columns="2" orderby="title"] PhD!
Here are some pictures from our recent trip up to Soda Springs and Sugarbowl for a weekend of skiing and scientific retreat. Dave also delivered the 2013 State of the Lab (SOL2013) address, highlighting that our exponential increase in restriction enzyme usage will require an entire RO1 dedicated to cloning by 2017. Better start writing those grants!