A huge congrats to Caleb on his new paper, "Identification of a minimal peptide tag for in vivo and in vitro loading of encapsulin," that was just published online in Biochemistry. Our lab is interested in the ability and effect of encapsulating proteins and other molecules inside protein compartments. Here, building off of beautiful work from Sutter et al., Caleb demonstrates that proteins can be loaded inside the minimal protein compartment from T. maritima known as encapsulin. Importantly, this work identifies a minimal targeting sequence, several different biochemical approaches for doing the loading, and a general strategy for obtaining large amounts of material.
Identification of a minimal peptide tag for in vivo and in vitro loading of encapsulin. Caleb Cassidy-Amstutz, Luke M Oltrogge, Catherine C Going, Antony Lee, Poh K Teng, David Quintanilla, Alexandra East-Seletsky, Evan R Williams, and David F Savage. Biochemistry. DOI: 10.1021/acs.biochem.6b00294