New paper from Sean on using recombineering for protein engineering

Check out Sean's new paper just published in ACS Synthetic Biology! Here we show that recombineering, a known method of bacterial genome engineering (pioneered first by Court and later by Wang/Isaacs/Church) can also be used for the reliable programmed construction of DNA libraries on plasmids. It turns out this can be a simple way to make libraries of protein expression plasmids for protein engineering studies. We then demonstrate the technique works by engineering a novel thermostable and brighter variant of the small fluorescent protein iLOV. Congrats Sean!